Journal: Organogenesis

Article Title: MicroRNA-214-3p Delivered by Bone Marrow Mesenchymal Stem Cells-Secreted Exosomes Affects Oxidative Stress in Alzheimer’s Disease Rats by Targeting CD151

doi: 10.1080/15476278.2025.2489673

Figure Lengend Snippet: Isolation and identification of bone MSCs and MSCs-exo. (A) flow cytometry analysis of MSCs-related surface markers, with pink representing the isotype control and cyan representing the specific marker; (B) Alizarin red staining detection of osteogenic differentiation of MSCs; (C) oil red O staining detection of adipogenic differentiation of MSCs; (D) TEM observation of exos; E: Western blot detection of exo-specific surface proteins. N = 3; each experiment was repeated three times.

Article Snippet: Cells were seeded in 6-well plates and cultured until 80% confluence, then induced with osteogenic induction medium (Cyagen, China) and adipogenic induction medium (Cyagen, China) for 14 days, with medium changes every 3 days.

Techniques: Isolation, Flow Cytometry, Control, Marker, Staining, Western Blot

Journal: Bioengineering & Translational Medicine

Article Title: A simple and efficient strategy for cell‐based and cell‐free‐based therapies in acute liver failure: hUCMSCs bioartificial liver

doi: 10.1002/btm2.10552

Figure Lengend Snippet: hUCMSCs acquisition and identification, and large‐scale expansion in hollow fiber bioreactor. (a) Schematic diagram of acquisition and identification, and large‐scale expansion of hUCMSCs, which were first expanded to 10 7 –10 8 in the culture flask, then inoculated into the hollow fiber bioreactor until amplified to 10 9 –10 10 ; (b) hUCMSCs were isolated by the tissue block method; (c) P5‐generation hUCMSCs morphology under an optical inverted microscope. (d) hUCMSCs differentiated into fat and stained with Oil red O (×200). (e) Osteogenic differentiation of hUCMSCs stained with alizarin red (×200). (f) Cartilage differentiation of hUCMSCs stained with safranin O (×200). (g) Flow cytometry showed that hUCMSCs highly expressed CD73, CD90, and CD105 (both ≥ 95%) but did not express CD34, CD45, CD19, CD11b, or HLA‐DR (both ≤ 5%); (h) The results of live and dead staining of hUCMSCs in the bioreactor; (i) hUCMSCs in the bioreactor under a light microscope; (j) Hollow‐fiber superfine structure in the bioreactor. (k) Adhesion of hUCMSCs to hollow fiber in the bioreactor. (l–m) Changes in GLU, Lac, and PO 2 in the bioreactor during hUCMSCs culture in (l) Group B and (m) Group C. GLU, glucose; Lac, lactate; PO 2 , oxygen partial pressure. Scale bar 200 μm.

Article Snippet: According to the manufacturer's instructions, adipogenic induction medium (STEMCELL, Catalog #05412), osteogenic induction medium (STEMCELL, Catalog #05465), and chondrogenic induction medium (STEMCELL, Catalog #05455) were used to culture hUCMSCs.

Techniques: Amplification, Isolation, Blocking Assay, Inverted Microscopy, Staining, Flow Cytometry, Light Microscopy

Journal: International Journal of Molecular Medicine

Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

doi: 10.3892/ijmm.2020.4556

Figure Lengend Snippet: Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, osteogenic growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.

Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Gene Expression, Incubation, Expressing, Control, Concentration Assay, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

doi: 10.3892/ijmm.2020.4556

Figure Lengend Snippet: Gene expression analysis of Col1a1, SPARC, TIMP1 and MMP1 at the mRNA level. Reverse transcription-quantitative PCR data for Col1a1, SPARC, TIMP1 and MMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in OGM or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. Col1a1, α 1 type 1 collagen; SPARC, secreted protein acidic and cysteine rich; TIMP1, tissue inhibitor of metalloproteinase 1; MMP1, metalloproteinase 1; OGM, osteogenic growth medium; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control, Concentration Assay

Journal: International Journal of Molecular Medicine

Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

doi: 10.3892/ijmm.2020.4556

Figure Lengend Snippet: Gene expression analysis of Runx2, BMP2, OCN and IBSP at the mRNA level. Reverse transcription-quantitative PCR data for Runx2, BMP2, OCN and IBSP genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, ***P≤0.001 and **** P≤0.0001. Runx2, runt-related transcription factor 2; BMP2, bone morphogenetic protein 2; OCN, osteocalcin; IBSP, integrin-binding sialoprotein; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control, Binding Assay, Concentration Assay

Journal: International Journal of Molecular Medicine

Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

doi: 10.3892/ijmm.2020.4556

Figure Lengend Snippet: Gene expression analysis of RANKL, OPG, RANKL/OPG and MMP1/TIMP1 at the mRNA level. Reverse transcription-quantitative PCR data for RANKL, OPG, RANKL/OPG and MMP1/TIMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. RANKL, receptor activator of nuclear factor κ-B ligand; OPG, osteoprotegerin; MMP1, metalloproteinase 1; TIMP1, tissue inhibitor of metalloproteinase 1; PRPEs, polyphenol-rich pomace extracts.

Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control